CONCENTRATION AND TIME DEPENDENT EFFECT OF ZINC CHLORIDE ON GLUTATHIONE LEVEL IN AQUEOUS MEDIUM

Rehan Zafar Paracha

Rehan Zafar Paracha Department of Pharm. Chemistry, Faculty of Pharmacy Gomal University D.I.Khan (KPK) Pakistan

Keywords: CONCENTRATION AND TIME DEPENDENT EFFECT OF ZINC CHLORIDE

Abstract

Zinc is important element, essential for supporting all life. Zinc prosthetic group is present in thousand of protein in the human body, that way one of that is so called zinc-finger. Moreover, dozens of kinds of cells in human-body secrete and their role in health and medicine is being studied. It is important to check and study in-vivo condition, influence of Zinc on GSH. In aqueous medium, the effect of zinc on the GSH level has been studied by Ellman’s method. This effect was concentration and time dependent and decrease was observed in both concentration and time dependent effect. The decrease in GSH level is due to the interaction of Zinc and GSH, and this oxidizes the GSH to their GSSG (Di-Sulphide) form with time and concentration. This study provided the effect of zinc metal on thiol/GSH level in-vitro, which may present a model of in-vivo reaction.