DETERMINATION OF GLUTATHIONE CONCENTRATION AFTER ITS INTERACTION WITH CADMIUM NITRATE TETRAHYDRATE BY USING ELLMAN’S MODIFIED METHOD

Abstract

Industrial waste is main source of environmental pollution especially of cadmium which is toxic to all organisms including humans because it interact with the thiol containing cellular components like Glutathione which is a tripeptide present in human body inside as well as outside the cells. So we have studied the interaction of Glutathione (GSH) with cadmium Nitrate tetrahydrate in aqueous medium by using modified Ellman’s method. For this purpose we studied the concentration and time dependent effect of cadmium nitrate tetrahydrate(CNT) on Glutathione in aqueous medium by using UV-visible spectrophotometer. Different concentrations of cadmium nitrate tetrahydrate were introduced to constant concentration of Glutathione to determine the affect of cadmium on concentration of GSH. It was found that higher concentration of Cd cause greater decrease in GSH concentration than lower concentration of cadmium nitrate tetrahydrate with the passage of time, at different time of intervals, there was a gradual depletion in the concentration of GSH that was indicating either binding of cadmium with GSH or conversion of GSH (reduced form of GSH) into GSSG ( oxidized form of GSH).