SIMULTANEOUS ESTIMATION AND VALIDATION OF CIS-/TRANS ISOMERS OF PERMETHRIN

Mukhtiar Hassan; Muhammad Akhlaq

Mukhtiar Hassan Department of Biochemistry, Hazara University, Mansehra, Pakistan
Muhammad Akhlaq Faculty of Pharmacy, Gomal University, D.I.Khan, Pakistan

Keywords: Permethrin, Cis/Trans Isomers, Estimation, Validation, High Performance Liquid Chromatography

Abstract

A sensitive and accurate high performance liquid chromatography assay (HPLC) technique was applied for determination of permethrin isomers. Cis-/trans-Permethrin isomers were separated at room temperature in raw material as well as pharmaceutical products such as lotions and creams without extraction. The calibration curve was found to be linear (r ≥ 0.9975) over the concentration range of 0.80-1.66 μm/ml. The samples were measured at 280 nm detection wavelength as internal standard of quantitaion. Mean percentage (%) recovery ± % relative standard deviation (RSD) ranged from 97.50 ± 1.36 to 100.08 ± 0.39. Within-day and between-day precision were also in acceptable range below 2%. The reported method for the simultaneous estimation of cis-/trans-permethrin isomers provides several advantages e.g simplicity, highly specificity, accuracy and very short run-cycle time.