DOSE RESPONSE CURVE OF PLANTS EXTRACTS AGAINST THE HUMAN PATHOGENS

Abstract

Plants have been significantly used since the ancient times due to the presence of diverse chemical compounds having therapeutic nature against various disorders and the infectious diseases of humans. Herbs and spices have been known for human diet but spices possess some allergic chemicals that’s why the search for drugs and dietary supplements derived from plants has more importance now a days. The present work shows that the antibacterial activity of methanolic garlic, ethanolic ginger and ethanolic neem extracts against the 21 human pathogens isolated from various specimens against various antibiotics. A disc impregnated in a mixture of Cefataxime (crude) was used as standard. The antibacterial activity was observed in all extracts and maximum effect of garlic on Staphylococcus aureus (90%), ginger on Staphylococcus aureus and Klebsiella pneumoniae (86 and 85 %) and neem on Staphylococcus aureus (95%) as compared to other test isolates respectively. Neem was reported as the most effective plant extract having least MIC with greater percentage of antibacterial activity as compared to the other test extracts when tested by disc diffusion and well diffusion methods. The minimum inhibitory concentration revealed 4, 6, 3, 3, 2 garlic, 6, 6, 5, 4, 3 garlic and 2, 3, 1, 2, 1 mg of neem extracts when suspended in 100 ml of the cell suspension 1x10-6 colony forming unit (cfu) respectively. It was concluded that Neem extract has strong and most effective antibacterial property as compared to ginger and garlic.